Microfluidic Compartmentalization Platforms for Single Cell Analysis

Biosensors

Jui-Yi Chen¹  Xuhao Luo¹  Abraham Lee¹  Marzieh Ataei² 

[1] Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA;Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA 92697, USA;

关键词: microvalves;    microwells;    droplets;    single-cell compartmentalization;    single-cell analysis;   

DOI  :  10.3390/bios12020058

来源: DOAJ

【 摘 要 】

Many cellular analytical technologies measure only the average response from a cell population with an assumption that a clonal population is homogenous. The ensemble measurement often masks the difference among individual cells that can lead to misinterpretation. The advent of microfluidic technology has revolutionized single-cell analysis through precise manipulation of liquid and compartmentalizing single cells in small volumes (pico- to nano-liter). Due to its advantages from miniaturization, microfluidic systems offer an array of capabilities to study genomics, transcriptomics, and proteomics of a large number of individual cells. In this regard, microfluidic systems have emerged as a powerful technology to uncover cellular heterogeneity and expand the depth and breadth of single-cell analysis. This review will focus on recent developments of three microfluidic compartmentalization platforms (microvalve, microwell, and microdroplets) that target single-cell analysis spanning from proteomics to genomics. We also compare and contrast these three microfluidic platforms and discuss their respective advantages and disadvantages in single-cell analysis.

【 授权许可】

Unknown