Electrochemical Immunosensor for Detection of IgY in Food and Food Supplements

Chemosensors

Emmanuele Ambrosi¹  Paolo Ugo¹  Chiara Gaetani¹  Ligia M. Moretto¹ 

[1] Department of Molecular Sciences and Nanosystems, University Ca’ Foscari of Venice, 30174 Venice, Italy;

关键词: immunoglobulin Y;    egg yolk;    nanoelectrodes ensembles;    electrochemical immunosensor;    food supplements;    SDS-PAGE;   

DOI  :  10.3390/chemosensors5010010

来源: DOAJ

【 摘 要 】

Immunoglobulin Y is a water-soluble protein present in high concentration in hen serum and egg yolk. IgY has applications in many fields, e.g., from food stuff to the mass production of antibodies. In this work, we have implemented an electrochemical immunosensor for IgY based on templated nanoelectrodes ensembles. IgY is captured by the templating polycarbonate and reacted with anti-IgY labeled with horseradish peroxidase. In the presence of H2O2 and methylene blue as the redox mediator, an electrocatalytic current is generated which scales with IgY concentration in the sample. After optimizing the extracting procedure, the immunosensor was applied for analysis of fresh eggs and food integrators. The data obtained with the biosensor were validated by SDS-PAGE and Western blot measurements.

【 授权许可】

Unknown