Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections

Memórias do Instituto Oswaldo Cruz

Gentil Arthur Bentes¹  Natália Maria Lanzarini¹  Lyana Rodrigues Pinto Lima¹  Pedro Paulo De Abreu Manso¹  Alexandre Dos Santos Da Silva¹  Sergio Da Silva E Mouta Junior¹  Juliana Rodrigues Guimarães¹  Marcia Terezinha Baroni De Moraes¹  Marcelo Pelajo-machado¹  Marcelo Alves Pinto¹ 

关键词: immunoglobulin Y;    hepatitis A virus;    indirect immunofluorescence;   

DOI  :  10.1590/0074-02760140457

来源: SciELO

【 摘 要 】

An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.

【 授权许可】

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