Long non-coding RNA LNC_000641 regulates pseudorabies virus replication

Veterinary Research

Linlin Fang¹  Juan Bai¹  Yanni Gao¹  Xing Liu¹  Ping Jiang¹  XianWei Wang¹ 

[1] Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University;

关键词: Pseudorabies virus;    Long non-coding RNAs (lncRNAs);    Anti-viral activity;    IFN-alpha;    JAK/STAT1;   

DOI  :  10.1186/s13567-021-00922-0

来源: DOAJ

【 摘 要 】

Abstract Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant altered expressions in 3D4/21 cells infected with PRV (ZJ01) were identified. Five lncRNAs upregulated in PRV-infected cells were verified in cells infected with different PRV strains by qRT-PCR. By down- and up-regulation of lnc641, the accelerating effect of lnc641 on PRV replication was confirmed. Furthermore, we found that lnc641 regulated PRV replication by inhibiting the JAK-STAT1 pathway. This study suggests that lnc641 could be a new host factor target for developing antiviral therapies against PRV infection.

【 授权许可】

Unknown