Long non-coding RNA LNC_000641 regulates pseudorabies virus replication

Veterinary Research

Linlin Fang¹  Xing Liu²  Yanni Gao²  XianWei Wang²  Ping Jiang²  Juan Bai² 

[1] Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, 210095, Nanjing, China;Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, 210095, Nanjing, China;Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, 225009, Yangzhou, China;

关键词: Pseudorabies virus;    Long non-coding RNAs (lncRNAs);    Anti-viral activity;    IFN-alpha;    JAK/STAT1;   

DOI  :  10.1186/s13567-021-00922-0

来源: Springer

【 摘 要 】

Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant altered expressions in 3D4/21 cells infected with PRV (ZJ01) were identified. Five lncRNAs upregulated in PRV-infected cells were verified in cells infected with different PRV strains by qRT-PCR. By down- and up-regulation of lnc641, the accelerating effect of lnc641 on PRV replication was confirmed. Furthermore, we found that lnc641 regulated PRV replication by inhibiting the JAK-STAT1 pathway. This study suggests that lnc641 could be a new host factor target for developing antiviral therapies against PRV infection.

【 授权许可】

CC BY