A Single-Cell RNA Expression Map of Human Coronavirus Entry Factors

Cell Reports

Vikas Bansal¹  Cédric Feschotte²  Manvendra Singh³ 

[1] Corresponding author;Biomedical Data Science and Machine Learning Group, German Center for Neurodegenerative Diseases, Tübingen 72076, Germany;Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA;

关键词: COVID-19;    SARS-CoV-2;    coronaviruses;    scRNA-seq;    viral receptors;    restriction factors;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: To predict the tropism of human coronaviruses, we profile 28 SARS-CoV-2 and coronavirus-associated receptors and factors (SCARFs) using single-cell transcriptomics across various healthy human tissues. SCARFs include cellular factors both facilitating and restricting viral entry. Intestinal goblet cells, enterocytes, and kidney proximal tubule cells appear highly permissive to SARS-CoV-2, consistent with clinical data. Our analysis also predicts non-canonical entry paths for lung and brain infections. Spermatogonial cells and prostate endocrine cells also appear to be permissive to SARS-CoV-2 infection, suggesting male-specific vulnerabilities. Both pro- and anti-viral factors are highly expressed within the nasal epithelium, with potential age-dependent variation, predicting an important battleground for coronavirus infection. Our analysis also suggests that early embryonic and placental development are at moderate risk of infection. Lastly, SCARF expression appears broadly conserved across a subset of primate organs examined. Our study establishes a resource for investigations of coronavirus biology and pathology.

【 授权许可】

Unknown