Phytochemical profiling and anti-aging activities of Euphorbia retusa extract: In silico and in vitro studies

Arabian Journal of Chemistry

Ahmed Gaara¹  Mansour Sobeh²  Abdelbaset M. Elgamal³  Mohamed A.O. Abdelfattah⁴  Mohamed A. El Raey⁵ 

[1] Corresponding authors.;College of Engineering and Technology, American University of the Middle East, Kuwait;Department of Chemistry of Microbial and Natural Products, National Research Centre, Dokki, Cairo 12622, Egypt;Department of Chemistry of Natural Compounds, Pharmaceutical Division, National Research Centre, Dokki, Cairo, Egypt;Department of Phytochemistry and Plant Systematics, Pharmaceutical Division, National Research Centre, Dokki, Cairo 12622, Egypt;

关键词: Euphorbia retusa;    Anti-aging;    Antioxidant;    Collagenase;    Elastase;    Hyaluronidase;   

DOI  :  

来源: DOAJ

【 摘 要 】

In this work, the phytochemical profiling of Euphorbia retusa aerial parts by HPLC-PDA-MS/MS revealed 21 secondary metabolites including phenolic acids, flavonoids, and their glycoside derivatives. The chemical structures of four isolated compounds (Esculetin, quercetin 3-O-β-D-glucuronide, kaempferol 3-O-β-D-glucuronide, and kaempferol 3-O-β-D-glucoside) were elucidated using mass spectrometry, 1H, and 13C NMR spectroscopy. Among the isolated compounds, kaempferol-3-O-glucuronide and esculetin were identified for the first time from the plant. An in silico molecular docking study showed the high potential of the identified compounds in the extract to bind to the active sites of four enzymes crucially involved in skin remodeling and aging processes. The in vitro assay confirmed the docking results against collagenase, elastase, hyaluronidase, and tyrosinase enzymes respectively. Among the four isolated compounds, kaempferol 3-O-glucoside was the highest active compound against the aforementioned enzymes. Moreover, the extract showed substantial antioxidant activity in DPPH assay. In conclusion, E. retusa is a substantial source for bioactive secondary metabolites with potential application in aging.

【 授权许可】

Unknown