The role of miR-29c/B7-H3 axis in children with allergic asthma

Journal of Translational Medicine

Xin Zhao¹  Canhong Zhu²  Yongdong Yan²  Li Huang²  Wei Ji²  Chuangli Hao²  Wujun Jiang²  Yuqing Wang²  Zhengrong Chen²  Xinxing Zhang²  Huiming Sun²  Wenjin Gu² 

[1] Department of General Surgery, The First Affiliated Hospital of Soochow University, Soochow University;Department of Respiratory Disease, Children’s Hospital of Soochow University, Soochow University;

关键词: Asthma;    Children;    MircoRNA;    B7-H3;    Th cell differentiation;   

DOI  :  10.1186/s12967-018-1590-8

来源: DOAJ

【 摘 要 】

Abstract Background MicroRNAs play roles in the pathogenesis of bronchial asthma. However, the mechanism of miR-29c in allergic asthma remains unclear. This study is to elucidate the regulation of Th cell differentiation by miR-29c in mononuclear macrophages. Methods A total of 52 children with asthma exacerbation and 26 children as controls were enrolled in the study. CD14+ monocytes were isolated from the peripheral blood. Differential expressions of microRNAs were evaluated using microarray analysis and miR-29c expression in monocytes was determined by qRT-PCR. The plasma B7-H3 was determined by ELISA. Transfection studies and luciferase reporter assay were performed to confirm target gene of miR-29c and its function. Results Compared to controls, 88 miRNAs in blood monocytes were up-regulated and 41 miRNAs down-regulated including miR-29c in asthma children. Children with asthma exacerbation had significantly lower level of miR-29c and higher level of plasma B7-H3 compared to controls (both P < 0.05). Functional studies based on luciferase reporter assay and immunofluorescence staining suggest that B7-H3 is the direct target of miR-29c and transfection anti-miR-29c into macrophages could enhance ROR-γt and GATA-3 expression in co-cultured CD4+ T cells and increase levels of IL-4 and IL-17 in supernatants. Conclusion The axis of miR-29c/B7-H3 plays an important role in children with asthma through regulating Th2/Th17 cell differentiation and may provide new targets for treatment of asthma.

【 授权许可】

Unknown