Plasticity-Related Gene 5 Is Expressed in a Late Phase of Neurodifferentiation After Neuronal Cell-Fate Determination

Frontiers in Cellular Neuroscience

Martin Witt¹  Danara Vonk²  Mark S. Hipp²  Ralf Rabus³  Lars Wöhlbrand³  Franziska Köper⁴  Torsten Plösch⁵  Axel Heep⁵  Anja U. Bräuer⁵  Isabel Gross⁷  Nicola Brandt⁷ 

[1] Department of Anatomy, University Medical Center Rostock, Rostock, Germany;Department of Biomedical Sciences of Cells and Systems, University Medical Center Groningen, University of Groningen, Groningen, Netherlands;General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University of Oldenburg, Oldenburg, Germany;Perinatal Neurobiology Research Group, School of Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany;Research Center Neurosensory Science, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany;Research Group Anatomy, School of Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany;School of Medicine and Health Sciences, Carl von Ossietzky University of Oldenburg, Oldenburg, Germany;

关键词: neurogenesis;    PRGs;    dendritic spines;    neurodifferentiation;    brain development;   

DOI  :  10.3389/fncel.2022.797588

来源: DOAJ

【 摘 要 】

During adult neurogenesis, neuronal stem cells differentiate into mature neurons that are functionally integrated into the existing network. One hallmark during the late phase of this neurodifferentiation process is the formation of dendritic spines. These morphological specialized structures form the basis of most excitatory synapses in the brain, and are essential for neuronal communication. Additionally, dendritic spines are affected in neurological disorders, such as Alzheimer’s disease or schizophrenia. However, the mechanisms underlying spinogenesis, as well as spine pathologies, are poorly understood. Plasticity-related Gene 5 (PRG5), a neuronal transmembrane protein, has previously been linked to spinogenesis in vitro. Here, we analyze endogenous expression of the PRG5 protein in different mouse brain areas, as well as on a subcellular level. We found that native PRG5 is expressed dendritically, and in high abundance in areas characterized by their regenerative capacity, such as the hippocampus and the olfactory bulb. During adult neurogenesis, PRG5 is specifically expressed in a late phase after neuronal cell-fate determination associated with dendritic spine formation. On a subcellular level, we found PRG5 not to be localized at the postsynaptic density, but at the base of the synapse. In addition, we showed that PRG5-induced formation of membrane protrusions is independent from neuronal activity, supporting a possible role in the morphology and stabilization of spines.

【 授权许可】

Unknown