A protocol for characterization of extremely preterm infant gut microbiota in double-blind clinical trials

STAR Protocols

Johanne E. Spreckels¹  Thomas Abrahamsson²  Maria C. Jenmalm³  Magalí Martí³ 

[1] Corresponding author;Department of Genetics, University Medical Centre Groningen, Groningen, the Netherlands;Department of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden;

关键词: Health Sciences;    Clinical Protocol;    Sequencing;    Microbiology;    Molecular Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: 16S rRNA gene sequencing enables microbial community profiling, but recovering fecal DNA from extremely premature infants is challenging. Here, we describe an optimized protocol for fecal DNA isolation, library preparation for 16S rRNA gene sequencing, taxonomy assignation, and statistical analyses. The protocol is complemented with a quantitative PCR for probiotic L. reuteri identification. This protocol describes how to characterize preterm infant gut microbiota and relate it to probiotic supplementation and clinical outcomes. It is customizable for other clinical trials.For complete details on the use and execution of this protocol, please refer to Martí et al. (2021) and Spreckels et al. (2021).

【 授权许可】

Unknown