Blue Whiting (Micromesistius poutassou) Protein Hydrolysates Increase GLP-1 Secretion and Proglucagon Production in STC-1 Cells Whilst Maintaining Caco-2/HT29-MTX Co-Culture Integrity

Marine Drugs

Snehal Gite¹  Jason Whooley¹  Leo Nunn²  Pádraigín A. Harnedy-Rothwell³  Richard J. FitzGerald³  Shauna Heffernan⁴  Nora M. O’Brien⁴  Linda Giblin⁵ 

[1] Bio-Marine Ingredients Ireland Ltd., Lough Egish Food Park, A75 WR82 Castleblaney, Ireland;Department of Biological Sciences, Munster Technological University, T12 P928 Cork, Ireland;Department of Biological Sciences, University of Limerick, V94 T9PX Limerick, Ireland;School of Food and Nutritional Sciences, University College Cork, T12 YN60 Cork, Ireland;Teagasc Food Research Centre, Moorepark, Fermoy, P61 C996 Cork, Ireland;

关键词: blue whiting;    protein hydrolysates;    satiety hormones;    GLP-1;    STC-1;    gut health;   

DOI  :  10.3390/md20020112

来源: DOAJ

【 摘 要 】

Inducing the feeling of fullness via the regulation of satiety hormones presents an effective method for reducing excess energy intake and, in turn, preventing the development of obesity. In this study, the ability of blue whiting soluble protein hydrolysates (BWSPHs) and simulated gastrointestinal digested (SGID) BWSPHs, to modulate the secretion and/or production of satiety hormones, such as glucagon-like peptide-1 (GLP-1), cholecystokinin (CCK) and peptide YY (PYY), was assessed in murine enteroendocrine STC-1 cells. All BWSPHs (BW-SPH-A to BW-SPH-F) (1.0% w/v dw) increased active GLP-1 secretion and proglucagon production in STC-1 cells compared to the basal control (Krebs–Ringer buffer) (p < 0.05). The signaling pathway activated for GLP-1 secretion was also assessed. A significant increase in intracellular calcium levels was observed after incubation with all BWSPHs (p < 0.05) compared with the control, although none of the BWSPHs altered intracellular cyclic adenosine monophosphate (cAMP) concentrations. The secretagogue effect of the leading hydrolysate was diminished after SGID. Neither pre- nor post-SGID hydrolysates affected epithelial barrier integrity or stimulated interleukin (IL)-6 secretion in differentiated Caco-2/HT-29MTX co-cultured cells. These results suggest a role for BWSPH-derived peptides in satiety activity; however, these peptides may need to be protected by some means to avoid loss of activity during gastrointestinal transit.

【 授权许可】

Unknown