Evaluating Adverse Effects of Inhaled Nanoparticles by Realistic In Vitro Technology

Nanomaterials

Martin Fierz¹  Heinz Burtscher¹  Marianne Geiser²  Natalie Jeannet² 

[1] Institute of Aerosol and Sensor Technology, University of Applied Sciences and Arts Northwestern Switzerland, Klosterzelgstrasse 2, 5210 Windisch, Switzerland;Institute of Anatomy, University of Bern, Baltzerstrasse 2, P.O. Box 922, 3012 Bern, Switzerland;

关键词: 3R;    aerosol;    air-liquid interface;    airway epithelia;    electrostatic deposition;    engineered nanoparticles;    in vitro;    NACIVT;    toxicology;    nanoparticles;   

DOI  :  10.3390/nano7020049

来源: DOAJ

【 摘 要 】

The number of daily products containing nanoparticles (NP) is rapidly increasing. NP in powders, dispersions, or sprays are a yet unknown risk for incidental exposure, especially at workplaces during NP production and processing, and for consumers of any health status and age using NP containing sprays. We developed the nano aerosol chamber for in vitro toxicity (NACIVT), a portable instrument for realistic safety testing of inhaled NP in vitro and evaluated effects of silver (Ag) and carbon (C) NP—which belong to the most widely used nanomaterials—on normal and compromised airway epithelia. We review the development, physical performance, and suitability of NACIVT for short and long-term exposures with air-liquid interface (ALI) cell cultures in regard to the prerequisites of a realistic in vitro test system for inhalation toxicology and in comparison to other commercially available, well characterized systems. We also review doses applied to cell cultures in vitro and acknowledge that a single exposure to realistic doses of spark generated 20-nm Ag- or CNP results in small, similar cellular responses to both NP types and that cytokine release generally increased with increasing NP dose.

【 授权许可】

Unknown