Olfactory receptor accessory proteins play crucial roles in receptor function and gene choice

eLife

Ian Davison¹  Helena You²  Kentaro Ikegami³  Yoshiro Ishimaru⁴  Masafumi Yohda⁴  Michael Ehlers⁴  Quiyi Chi⁴  Ruchira Sharma⁴  Ming-Shan Chien⁴  Momoka Kubota⁴  Hiroaki Matsunami⁵ 

[1] Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;Tokyo University of Agriculture and Technology, Tokyo, Japan;Department of Biology, Boston University, Boston, United States;Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, United States;Tokyo University of Agriculture and Technology, Tokyo, Japan;

关键词: GPCR;    UPR;    chaperone;    olfaction;    odorant;    epigenetic;   

DOI  :  10.7554/eLife.21895

来源: DOAJ

【 摘 要 】

Each of the olfactory sensory neurons (OSNs) chooses to express a single G protein-coupled olfactory receptor (OR) from a pool of hundreds. Here, we show the receptor transporting protein (RTP) family members play a dual role in both normal OR trafficking and determining OR gene choice probabilities. Rtp1 and Rtp2 double knockout mice (RTP1,2DKO) show OR trafficking defects and decreased OSN activation. Surprisingly, we discovered a small subset of the ORs are expressed in larger numbers of OSNs despite the presence of fewer total OSNs in RTP1,2DKO. Unlike typical ORs, some overrepresented ORs show robust cell surface expression in heterologous cells without the co-expression of RTPs. We present a model in which developing OSNs exhibit unstable OR expression until they choose to express an OR that exits the ER or undergo cell death. Our study sheds light on the new link between OR protein trafficking and OR transcriptional regulation.

【 授权许可】

Unknown