In vitro study of sodium butyrate on soyasaponin challenged intestinal epithelial cells of turbot (Scophthalmus maximus L.) refer to inflammation, apoptosis and antioxidant enzymes

Fish and Shellfish Immunology Reports

Weihao Ou¹  Wenbing Zhang¹  Yanjiao Zhang¹  Qinghui Ai²  Kangsen Mai²  Guijuan Yu² 

[1] the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, China;;The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) &

关键词: Sodium butyrate;    Soyasaponins;    Inflammation;    Apoptosis;    MAPKs;    Turbot;   

DOI  :  

来源: DOAJ

【 摘 要 】

The study is aimed to investigate the protective effect and potential mechanisms of sodium butyrate (NaBT) on soyasaponins (SA) induced intestinal epithelial cells (IECs) injury in vitro. The primary IECs of turbot were developed and treated with 0.4, 1 and 4 mM NaBT in the presence of 0.4 mg/mL SA for 6 h to explore the protective effects of NaBT. The results showed that the addition of NaBT significantly down-regulated gene expression of inflammatory cytokine TNF-α, IL-1β and IL-8, pro-apoptosis relevant gene BAX, caspase-3, caspase-7 and caspase-9 induced by SA, while up-regulated anti-apoptosis gene Bcl-2. SA stimulation did not induce reactive oxygen species production, but elevated gene expression of antioxidant enzyme heme oxygenase-1 and superoxide dismutase. Moreover, the gene expression of those antioxidant enzyme was further up-regulated in NaBT groups. Furthermore, NaBT supplementation decreased the acid phosphatase and alkaline phosphatase activities and suppressed phosphorylation of p38 and c-Jun N-terminal kinase (JNK). In conclusion, NaBT could mitigate SA-induced inflammation and apoptosis and elevate gene expression of antioxidant enzymes on IECs of turbot and p38 and JNK signaling pathway participated in those processes.

【 授权许可】

Unknown