Expression Patterns of Three Important Hormone Genes and Respiratory Metabolism in Antheraea pernyi during Pupal Diapause under a Long Photoperiod

Insects

Yu-Meng Bian¹  Li Qin¹  Xiao-Xia Duan¹  Yong Wang¹  Qi Wang¹  Yu-Tong Luo¹  De-Yi Wang¹  Wei Liu¹  Yao-Ting Zhang² 

[1] College of Bioscience and Biotechnology, Shenyang Agricultural University, Insect Resource Research Center for Engineering and Technology of Liaoning Province, Shenyang 110866, China;Henan Academy of Sericulture Science, Zhengzhou 450008, China;

关键词: Antheraea pernyi;    diapause;    prothoracicotropic hormone;    ecdysis triggering hormone;    eclosion hormone;   

DOI  :  10.3390/insects12080699

来源: DOAJ

【 摘 要 】

The Chinese oak silkworm is commonly used in pupal diapause research. In this study, a long photoperiod was used to trigger pupal diapause termination. Genes encoding three hormones, namely prothoracicotropic hormone (PTTH), ecdysis triggering hormone (ETH), and eclosion hormone (EH), were studied. Additionally, ecdysteroids (mainly 20-hydroxyecdysone, 20E) were quantified by HPLC. Pupal diapause stage was determined by measuring respiratory intensity. The pupae enter a low metabolic rate, which starts approximately 1 month after pupal emergence. ApPTTH expression showed a small increase at 14 days and then a larger increase from 35 days under the long photoperiod treatment. A similar pattern was observed for the titer of 20E in the hemolymph. However, ApETH expression later increased under the long photoperiod treatment (42 days) just before eclosion. Moreover, ApEH expression increased from 21 to 35 days, and then decreased before ecdysis. These results suggest that hormone-related gene expression is closely related to pupal development. Our study lays a foundation for future diapause studies in A. pernyi.

【 授权许可】

Unknown