Pupal behavior emerges from unstructured muscle activity in response to neuromodulation in Drosophila

eLife

Hari Shroff¹  Carson C Chow²  Matthew Houpert³  Adama Berndt³  Robert L Scott³  Snehashis Roy³  Benjamin H White³  Amicia D Elliott⁴ 

[1] National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, United States;National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States;National Institute of Mental Health, National Institutes of Health, Bethesda, United States;National Institute of Mental Health, National Institutes of Health, Bethesda, United States;National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, United States;

关键词: neural circuit;    computational ethology;    bursicon;    CCAP;    ecdysis;    ecdysis triggering hormone;   

DOI  :  10.7554/eLife.68656

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Identifying neural substrates of behavior requires defining actions in terms that map onto brain activity. Brain and muscle activity naturally correlate via the output of motor neurons, but apart from simple movements it has been difficult to define behavior in terms of muscle contractions. By mapping the musculature of the pupal fruit fly and comprehensively imaging muscle activation at single-cell resolution, we here describe a multiphasic behavioral sequence in Drosophila. Our characterization identifies a previously undescribed behavioral phase and permits extraction of major movements by a convolutional neural network. We deconstruct movements into a syllabary of co-active muscles and identify specific syllables that are sensitive to neuromodulatory manipulations. We find that muscle activity shows considerable variability, with sequential increases in stereotypy dependent upon neuromodulation. Our work provides a platform for studying whole-animal behavior, quantifying its variability across multiple spatiotemporal scales, and analyzing its neuromodulatory regulation at cellular resolution.

【 授权许可】

CC BY