Morinagadepsin, a Depsipeptide from the Fungus Morinagamyces vermicularis gen. et comb. nov

Microorganisms

Karen Harms¹  Frank Surup¹  Marc Stadler¹  Yasmina Marin-Felix¹  AlbertoMiguel Stchigel² 

[1] Department Microbial Drugs, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany;Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili, C/ Sant Llorenç 21, Tarragona, 43201 Reus, Spain;

关键词: cytotoxicity;    depsipeptide;    Morinagamyces;    morinagadepsin;    Schizotheciaceae;    secondary metabolites;   

DOI  :  10.3390/microorganisms9061191

来源: DOAJ

【 摘 要 】

The new genus Morinagamyces is introduced herein to accommodate the fungus Apiosordaria vermicularis as inferred from a phylogenetic study based on sequences of the internal transcribed spacer region (ITS), the nuclear rDNA large subunit (LSU), and partial fragments of ribosomal polymerase II subunit 2 (rpb2) and β-tubulin (tub2) genes. Morinagamyces vermicularis was analyzed for the production of secondary metabolites, resulting in the isolation of a new depsipeptide named morinagadepsin (1), and the already known chaetone B (3). While the planar structure of 1 was elucidated by extensive 1D- and 2D-NMR analysis and high-resolution mass spectrometry, the absolute configuration of the building blocks Ala, Val, and Leu was determined as -l by Marfey’s method. The configuration of the 3-hydroxy-2-methyldecanyl unit was assigned as 22R,23R by J-based configuration analysis and Mosher’s method after partial hydrolysis of the morinagadepsin to the linear derivative compound 2. Compound 1 showed cytotoxic activity against the mammalian cell lines KB3.1 and L929, but no antimicrobial activity against the fungi and bacteria tested was observed, while 2 was inactive. Compound 3 was weakly cytotoxic against the cell line L929, but did not show any antimicrobial activity.

【 授权许可】

Unknown