Self-Assembling β-Glucan Nanomedicine for the Delivery of siRNA

Biomedicines

Daejin Min¹  Hojeong Jeon²  Yong Woo Cho³  Jangsun Hwang⁴  Semi Yoon⁵  Kyungwoo Lee⁵  Yonghyun Choi⁵  Jonghoon Choi⁵  Jaehee Jang⁵ 

[1] BioScience Lab, Basic Research & Innovation Division, R&D Unit, AMOREPACIFIC, Yongin-si 17074, Korea;Center for Biomaterials, Korea Institute of Science and Technology, Seoul 02792, Korea;Department of Chemical Engineering, Hanyang University, Ansan-si 15588, Korea;School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore;School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea;

关键词: beta-glucan;    self-assembly;    nanomedicine;    siRNA;    gene delivery;   

DOI  :  10.3390/biomedicines8110497

来源: DOAJ

【 摘 要 】

We aimed to design and manufacture a transporter capable of delivering small interfering RNAs (siRNAs) into the skin without causing any damage. β-glucans are unique chiral polysaccharides with well-defined immunological properties and supramolecular wrapping ability. However, the chiral properties of these polymers have hardly been applied in drug delivery systems. In this study, β-glucan nanoparticles were designed and manufactured to deliver genetic material to the target cells. The β-glucan molecules were self-assembled with an siRNA into nanoparticles of 300–400 nm in diameter via a conformational transition process, in order to construct a gene delivery system. The assembled gene nanocarriers were associated with high gene-loading ability. The expression and efficiency of siRNA were verified after its delivery via β-glucan. Our results provide evidence that β-glucan nanoparticles can be effectively used to deliver siRNA into the cells.

【 授权许可】

Unknown