Investigation of Active Anti-Inflammatory Constituents of Essential Oil from Pinus koraiensis (Sieb. et Zucc.) Wood in LPS-Stimulated RBL-2H3 Cells

Biomolecules

Mi-Jin Park¹  Ji-Yoon Yang¹  Ki-Joong Kim²  Chang-Deuk Eom³  Won-Sil Choi⁴ 

[1] Division of Forest Industrial Materials, Department of Forest Products and Industry, National Institute of Forest Science, Seoul 02455, Korea;Division of Life Sciences, School of Life Sciences, Korea University, Seoul 02841, Korea;Division of Wood Industry, Department of Forest Products and Industry, National Institute of Forest Science, Seoul 02455, Korea;National Instrumentation Center for Environmental Management, Seoul National University, Seoul 08826, Korea;

关键词: Pinus koraiensis;    essential oil;    anti-inflammatory;    active compound;    α-pinene;    β-pinene;   

DOI  :  10.3390/biom11060817

来源: DOAJ

【 摘 要 】

In a previous study, we demonstrated the anti-inflammatory activity of the essential oil extracted from Korean pine (Pinus koraiensis, Sieb. et Zucc.) wood. This study aims to investigate the active anti-inflammatory constituents of P. koraiensis oil. The essential oil was extracted from P. koraiensis wood by hydrodistillation and was divided into six fractions (A–F) through fractional distillation. Then, the anti-inflammatory activities of the fractions (A–F) were determined. Fractions A and F markedly downregulated the production of pro-inflammatory cytokines as well as the secretion of β-hexosaminidase in lipopolysaccharide (LPS)-stimulated RBL-2H3 cells. The main constituents of the active anti-inflammatory A and F fractions were (+)-α-pinene, (−)-β-pinene, (+)-α-terpineol, 3-carene, (+)-limonene, and longifolene. These six single compounds decreased the expression of inflammatory-related genes (i.e., IL-4 and IL-13) as well as the secretion of β-hexosaminidase in LPS-stimulated RBL-2H3 cells. (+)-α-Pinene, (−)-β-pinene, (+)-α-terpineol, and longifolene exhibited the strongest effects; these effects were comparable to those of the positive control (i.e., dexamethasone). The findings indicate that the interactions between these components exhibit potential for the management and/or treatment of inflammatory conditions as well as base structures for the development of novel anti-inflammatory drugs.

【 授权许可】

Unknown