A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients

STAR Protocols

Lisa F.P. Ng¹  Laurent Renia²  Yun Shan Goh² 

[1] Corresponding author;Infectious Diseases Laboratories (ID Labs), Agency for Science, Technology and Research (A∗STAR), Immunos, Biopolis, Singapore 138648, Singapore;

关键词: Cell Biology;    Flow Cytometry/Mass Cytometry;    Health Sciences;    Immunology;    Molecular Biology;    Antibody;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein.For complete details on the use and execution of this protocol, please refer to Goh et al. (2021).

【 授权许可】

Unknown