Detection of CD8+ T cell-mediated immune responses to bacterial infection in mice

STAR Protocols

Gen Li¹  Jiawen Zhou¹  Jun Wu²  Dan Li²  Peng Jiang²  Zhongjun Dong³ 

[1] Tsinghua-Peking Center for Life Sciences, Beijing 100084, China;School of Life Sciences, Tsinghua University, Beijing 100084, China;School of Medicine and Institute for Immunology, Tsinghua University, Beijing 100084, China;

关键词: Cell Biology;    Flow Cytometry/Mass Cytometry;    Immunology;    Microbiology;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Efficient activation of CD8+ T cells is critical for bacterial resistance and eradicating malignancy in the body. Here, we present a step-by-step protocol to use Listeria monocytogenes expressing OVA (LmOVA) to stimulate endogenous CD8+ T cells. We describe the steps for adoptive transfer of OT-I CD8+ T cells to CD45.1 mice and then detail the steps for detection of the antigen-specific CD8+ T cells in response to LmOVA.For complete details on the use and execution of this protocol, please refer to Wu et al. (2021).

【 授权许可】

Unknown