Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages

Journal of Functional Foods

Johura Ansary¹  Francesca Giampieri²  Adelaida Esteban-Muñoz³  Maurizio Battino³  José M. Alvarez-Suarez⁴  Danila Cianciosi⁵  Denise Quinzi⁵  María D. Navarro-Hortal⁵  Adolfo Amici⁵  José Luis Quiles⁶  Tamara Y. Forbes-Hernandez⁷ 

[1] King Fahd Medical Research Center, King Abdulaziz University, 21589 Jeddah, Saudi Arabia;Research Group on Food, Nutritional Biochemistry and Health, Universidad Europea del Atlántico, 39011 Santander, Spain;Biomedical Research Centre, Institute of Nutrition and Food Technology “José Mataix Verdú”, Department of Physiology, Faculty of Pharmacy, University of Granada, Avda. del Conocimiento s/n. 18100 Armilla, Granada, Spain;Departamento de Ingeniería en Alimentos, Colegio de Ciencias e Ingenierías, Universidad San Francisco de Quito, Quito 170157, Ecuador;Department of Clinical Sciences, Polytechnic University of Marche, Ancona 60131, Italy;Department of Nutrition and Bromatology, University of Granada. 18071 Granada, Spain;Nutrition and Food Science Group, Department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo - Vigo, Spain;

关键词: Honey;    Inflammation;    Mitochondrial respiration;    Apoptosis;    Wound healing;    Antioxidant enzymes;   

DOI  :  

来源: DOAJ

【 摘 要 】

The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters.

【 授权许可】

Unknown