GraS is critical for chloroplast development and affects yield in rice

Journal of Integrative Agriculture

Hao-hua HE¹  Jun-ru FU²  Hui-ying HAO³  Jian-ping WANG³  Zhou HUANG³  Jin-peng HE³  Jie XU³  Zhi-xuan DU³  Hai-hui FU³ 

[1] Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education/Collaboration Center for Double-season Rice Modernization Production, Jiangxi Agricultural University/Research Center of Super Rice Engineering and Technology, Jiangxi Province, Nanchang 330045, P.R.China;Correspondence FU Jun-ru;Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education/Collaboration Center for Double-season Rice Modernization Production, Jiangxi Agricultural University/Research Center of Super Rice Engineering and Technology, Jiangxi Province, Nanchang 330045, P.R.China;

关键词: GraS;    bioinformatics;    candidate gene;    chlorophyll;    leaf color;    rice;   

DOI  :  

来源: DOAJ

【 摘 要 】

Leaf color has been considered an important agronomic trait in rice (Oryza sativa L.) for a long time. The changes in leaf color affect the yield of rice. In this study, a green-revertible albino (graS) mutant was isolated from a 60Co-gamma-irradiated mutant pool of indica cultivar Guangzhan 63-4S. The fine mapping indicated that graS mutant was mapped to chromosome 1, and was located in a confined region between markers ab134 and InDel 8 with genetic distances of 0.11 and 0.06 cM, respectively. Based on the annotation results, four open reading frames (ORFs) were predicted in this region. Sequence analysis revealed that LOC_Os01g55974 had a 2-bp nucleotide insertion (AA) in the coding region that led to premature termination at the 324th base. Sequence analysis and expression analysis of related genes indicated that LOC_Os01g55974 is the candidate gene of GraS. We studied the genome and protein sequences of LOC_Os01g55974, and the data showed that GraS contains a deoxycytidine deaminase domain, which was expressed ubiquitously in all tissues. Further investigation indicated that GraS plays an essential role in the regulation of chloroplast biosynthesis, photosynthetic capacity and yield. Moreover, leaf color mutant can be used as an effective marker for the purity of breeding and hybridization.

【 授权许可】

Unknown