Safe Administration of Carbon Nanotubes by Intravenous Pathway in BALB/c Mice

Nanomaterials

Erasmo Orrantia-Borunda¹  Ernesto Ramos-Martínez²  David Chávez-Flores³  Carmen González-Horta³  JoséJesús Guzmán-Mendoza³  Pilardel Carmen Hernández-Rodríguez³  Blanca Sánchez-Ramírez³  SilviaLorena Montes-Fonseca⁴ 

[1] Centro de Investigación en Materiales Avanzados (CIMAV), Miguel de Cervantes 120, Complejo Industrial Chihuahua, Chihuahua CP 31136, Mexico;Departamento de Anatomía Patológica del Hospital Ángeles Chihuahua. Av. Hacienda del Valle No. 7120, Chihuahua CP 31217, Mexico;Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua. Circuito No. 1 Campus Universitario II, Chihuahua CP 31125, Mexico;Instituto Tecnológico de Monterrey Campus Chihuahua, Heroico Colegio Militar 4700, Col. Nombre de Dios, Chihuahua CP 31300, Mexico;

关键词: carbon nanotubes;    toxicity;    mice;    renal damage;    lung damage;    nanotoxicology;   

DOI  :  10.3390/nano10020400

来源: DOAJ

【 摘 要 】

Carbon nanotubes (CNTs) are nanomaterials with multiple possible uses as drug carriers or in nanovaccine development. However, the toxicity of CNTs administered intravenously in in vivo models has not been fully described to date. This work aimed to evaluate the toxic effect of pristine multi-walled CNTs (UP-CNTs), purified (P-CNTs), or CNTs functionalized with fluorescein isothiocyanate (FITC-CNTs) administered by intravenous injection in BALB/c mice. Biochemical and histopathological parameters were analyzed at 1, 14, 29, and 60 days post-exposure. Pristine CNTs were the most toxic nanoparticles in comparison with P-CNTs or FITC-CNTs, increasing serum AST (≈ 180%), ALT (≈ 300%), and LDH (≈ 200%) levels at one day post-exposure. The urea/creatinine ratio suggested pre-renal injury at the 14th day accompanied of extensive lesions in kidneys, lungs, and liver. Biochemical and histological findings in mice exposed to P-CNTs had not significant differences compared to the controls. A lower toxic effect was detected in animals exposed to FITC-CNTs which was attributable to FITC toxicity. These results demonstrate that the purification process of CNTs reduces in vivo toxicity, and that toxicity in functionalized CNTs is dependent on the functionalized compound. Therefore, P-CNTs are postulated as potential candidates for safe biomedical applications using an intravenous pathway.

【 授权许可】

Unknown