Neuropeptide F inhibits dopamine neuron interference of long-term memory consolidation in Drosophila

iScience

Cheng-Chang Lien¹  J. Steven de Belle²  Ann-Shyn Chiang³  Ju-Yun Weng⁴  Tim Tully⁴  Hsuan-Wen Lin⁴  Chun-Chao Chen⁴  Mohammed Bin Abubaker⁴  Chung-Chuan Lo⁴  Kuan-Lin Feng⁵  Ching-Che Charng⁶ 

[1] Department of Psychological Sciences, University of San Diego, San Diego, CA 92110, USA;Institute of Systems Neuroscience and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan;School of Life Sciences, University of Nevada, Las Vegas, NV 89154, USA;Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan;Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan;Institute of Systems Neuroscience and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan;

关键词: Biological sciences;    Neuroscience;    Behavioral neuroscience;    Cognitive neuroscience;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Long-term memory (LTM) formation requires consolidation processes to overcome interfering signals that erode memory formation. Olfactory memory in Drosophila involves convergent projection neuron (PN; odor) and dopaminergic neuron (DAN; reinforcement) input to the mushroom body (MB). How post-training DAN activity in the posterior lateral protocerebrum (PPL1) continues to regulate memory consolidation remains unknown. Here we address this question using targeted transgenes in behavior and electrophysiology experiments to show that (1) persistent post-training activity of PPL1-α2α′2 and PPL1-α3 DANs interferes with aversive LTM formation; (2) neuropeptide F (NPF) signaling blocks this interference in PPL1-α2α′2 and PPL1-α3 DANs after spaced training to enable LTM formation; and (3) training-induced NPF release and neurotransmission from two upstream dorsal-anterior-lateral (DAL2) neurons are required to form LTM. Thus, NPF signals from DAL2 neurons to specific PPL1 DANs disinhibit the memory circuit, ensuring that periodic events are remembered as consolidated LTM.

【 授权许可】

Unknown