Antimicrobial Activity of the Iron-Chelator, DIBI, against Multidrug-Resistant Canine Methicillin-Susceptible Staphylococcus pseudintermedius: A Preliminary Study of Four Clinical Strains

Pathogens

Giuseppe Iovane¹  Francesca Paola Nocera¹  Luisa De Martino¹  Bruce E. Holbein² 

[1] Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy;Fe Pharmaceuticals (Canada) Inc. (Formerly Chelation Partners Inc.), 58 The Labs at Innovacorp, Life Sciences Research Institute, 1344 Summer Street, Halifax, NS B3H 0A8, Canada;

关键词: antimicrobial resistance;    Staphylococcus pseudintermedius;    iron chelator;    DIBI;   

DOI  :  10.3390/pathogens11060656

来源: DOAJ

【 摘 要 】

Staphylococcus pseudintermedius is an important opportunistic pathogen causing various infections in dogs. Furthermore, it is an emerging zoonotic agent and both multidrug-resistant methicillin-resistant S. pseudintermedius (MRSP) as well as methicillin-susceptible (MSSP) strains represent an important therapeutic challenge to veterinary medicine and pose a potential threat to human health. We tested representative S. pseudintermedius clinical strains from dogs suffering from otitis externa for their susceptibilities to a panel of 17 antimicrobials compared to DIBI. DIBI, unlike antibiotics, is a novel water-soluble hydroxypyridinone-containing iron-chelating agent that deprives microbes of growth-essential iron and has been previously shown to inhibit methicillin-resistant Staphylococcus aureus (MRSA). We also characterised the strains according to whether they harbour key antibiotic resistance genes. The strains each displayed multiple antimicrobial resistance patterns; all were negative for the mecA gene and possessed the tetK and tetM genes, but they varied as to their possession of the ermB gene. However, all the isolates had similar susceptibility to DIBI with low MICs (2 µg/mL or 0.2 µM). Because the four MSSPs were equally susceptible to DIBI, subject to confirmation with additional strains, this could provide a potential non-antibiotic, anti-infective alternative approach for the treatment of antimicrobial-resistant canine S. pseudintermedius otitis.

【 授权许可】

Unknown