In Vitro Tests of FDM 3D-Printed Diclofenac Sodium-Containing Implants

Molecules

Marianna Zichar¹  Ildikó Papp¹  Mónika Béres²  Zoltán Ujhelyi³  Ferenc Fenyvesi³  Pálma Fehér³  Vecsernyés Miklós³  Ádám Haimhoffer³  Petra Arany³  Judit Váradi³  Ildikó Bácskay³  Gábor Vasvári³  Rudolf Gesztelyi⁴  Máté Csontos⁵  István Budai⁶  Géza Regdon⁷  János Elek⁸ 

[1] Department of Computer Graphics and Image Processing, Faculty of Informatics, University of Debrecen, Kassai út 26, H-4028 Debrecen, Hungary;Department of Medical Imaging, University of Debrecen, Nagyerdei Krt. 98, H-4032 Debrecen, Hungary;Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Debrecen, Nagyerdei Körút 98, H-4032 Debrecen, Hungary;Department of Pharmacology and Pharmacotherapy, University of Debrecen, Nagyerdei Körút 98, H-4032 Debrecen, Hungary;Department of Physical Chemistry, Faculty of Science and Technology, University of Debrecen, Egyetem tér 1, H-4032 Debrecen, Hungary;Faculty of Engineering, University of Debrecen, Ótemető utca 2-4, H-4028 Debrecen, Hungary;Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary;Science Port Kft., Varró utca 21, H-5300 Karcag, Hungary;

关键词: personalized medicine;    implants;    3D printing;    FDM;    dissolution tests;    cytotoxicity;   

DOI  :  10.3390/molecules25245889

来源: DOAJ

【 摘 要 】

One of the most promising emerging innovations in personalized medication is based on 3D printing technology. For use as authorized medications, 3D-printed products require different in vitro tests, including dissolution and biocompatibility investigations. Our objective was to manufacture implantable drug delivery systems using fused deposition modeling, and in vitro tests were performed for the assessment of these products. Polylactic acid, antibacterial polylactic acid, polyethylene terephthalate glycol, and poly(methyl methacrylate) filaments were selected, and samples with 16, 19, or 22 mm diameters and 0%, 5%, 10%, or 15% infill percentages were produced. The dissolution test was performed by a USP dissolution apparatus 1. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide dye (MTT)-based prolonged cytotoxicity test was performed on Caco-2 cells to certify the cytocompatibility properties. The implantable drug delivery systems were characterized by thermogravimetric and heatflow assay, contact angle measurement, scanning electron microscopy, microcomputed tomography, and Raman spectroscopy. Based on our results, it can be stated that the samples are considered nontoxic. The dissolution profiles are influenced by the material properties of the polymers, the diameter, and the infill percentage. Our results confirm the potential of fused deposition modeling (FDM) 3D printing for the manufacturing of different implantable drug delivery systems in personalized medicine and may be applied during surgical interventions.

【 授权许可】

Unknown