Half-life determination of inorganic-organic hybrid nanomaterials in mice using laser-induced breakdown spectroscopy

Journal of Advanced Research

Lianbo Guo¹  Qianyuan He²  Honglin Jin²  Zhanjie Zhang²  Fagang Jiang³  Deng Zhang⁴  Feng Chen⁴  Ziqian Sheng⁴  Yanwu Chu⁴ 

[1] Corresponding authors.;Cancer Center, Union Hospital, TongjiMedical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China;Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Wuhan National Laboratory for Optoelectronics (WNLO), Huazhong University of Science and Technology, Wuhan, Hubei 430074, China;

关键词: Manganese dioxide-bovine serum albumin;    Boehmite-bovine serum albumin;    Laser-induced breakdown spectroscopy;    Half-life detection;   

DOI  :  

来源: DOAJ

【 摘 要 】

Inorganic or inorganic-organic hybrid nanomaterials have great potential for applications in the biomedical fields. Biological half-life is an essential pharmacokinetic parameter for these materials to function in vivo. Compared to inductively coupled plasma mass spectrometry (ICP-MS), which is the gold standard, laser-induced breakdown spectroscopy (LIBS) is a faster and more efficient elemental detection method. We investigated an efficient way to quantify the metabolic rate using LIBS. Nanoparticle platforms, such as manganese dioxide-bovine serum albumin (MnO2-BSA) or boehmite-bovine serum albumin (AlO(OH)-BSA) were injected into mice through intravenous administration for LIBS spectrum acquisition. First, the spectral background was corrected using the polynomial fitting method; The spectral interference was eliminated by Lorentz fitting for each LIBS spectrum simultaneously. The support vector regression (SVR) was then used for LIBS quantitative analyses. Finally, the LIBS results were compared with the ICP-MS ones. The half-lives of MnO2-BSA calculated by LIBS and ICP-MS were 2.49 and 2.42 h, respectively. For AlO(OH)-BSA, the half-lives detected by LIBS and ICP-MS were 3.46 and 3.57 h, respectively. The relative error of LIBS is within 5% compared to ICP-MS. The results demonstrate that LIBS is a valuable tool for quantifying the metabolic rates with a high degree of accuracy.

【 授权许可】

Unknown