Microbiome Remodeling via the Montmorillonite Adsorption-Excretion Axis Prevents Obesity-related Metabolic Disorders

EBioMedicine

Jin Liu¹  Pengfei Xu¹  Jialin Wang¹  Fan Hong¹  Shu Dai¹  Sheng Wang¹  Yonggong Zhai¹  Xi Jin¹  Jing Wang²  Fang Wang³  Yusheng Cong⁴ 

[1] Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China;Department of Biology Science and Technology, Baotou Teacher's College, Baotou 014030, China;Department of Medical Genetics and Cell Biology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 511436, China;Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou 311121, China;

关键词: Montmorillonite;    Obesity;    Metabolic disorders;    Free fatty acids;    Endotoxins;    Gut microbiota;   

DOI  :  10.1016/j.ebiom.2017.01.019

来源: DOAJ

【 摘 要 】

Obesity and its related metabolic disorders are closely correlated with gut dysbiosis. Montmorillonite is a common medicine used to treat diarrhea. We have previously found that dietary lipid adsorbent-montmorillonite (DLA-M) has an unexpected role in preventing obesity. The aim of this study was to further investigate whether DLA-M regulates intestinal absorption and gut microbiota to prevent obesity-related metabolic disorders. Here, we show that DLA-M absorbs free fatty acids (FFA) and endotoxins in vitro and in vivo. Moreover, the combination of fluorescent tracer technique and polarized light microscopy showed that DLA-M crystals immobilized BODIPY® FL C16 and FITC-LPS, respectively, in the digestive tract in situ. HFD-fed mice treated with DLA-M showed mild changes in the composition of the gut microbiota, particularly increases in short-chain fatty acids (SCFA)-producing Blautia bacteria and decreases in endotoxin-producing Desulfovibrio bacteria, these changes were positively correlated with obesity and inflammation. Our results indicated that DLA-M immobilizes FFA and endotoxins in the digestive tract via the adsorption-excretion axis and DLA-M may potentially be used as a prebiotic to prevent intestinal dysbiosis and obesity-associated metabolic disorders in obese individuals.

【 授权许可】

Unknown