Short communication. First report of Eggplant mottled dwarf virus in China rose in southern Spain

Spanish Journal of Agricultural Research

I. M. Fortes¹  J. Navas-Castillo¹  F. Villanueva¹  G. Parrella²  B. Greco²  A. De Stradis³ 

[1] Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” (IHSM-UMA-CSIC), Estación Experimental "La Mayora", 29750 Algarrobo-Costa, Málaga;Istituto per la Protezione delle Piante del CNR, UOS di Portici, Via Università 133, 80055 Portici (NA);Istituto per la Virologia Vegetale del CNR, UOS di Bari, Via Amendola 165/a, 70126 Bari;

关键词: Hibiscus rosa-sinensis;    EMDV;    Nucleorhabdovirus;    molecular probe;    ELISA;   

DOI  :  10.5424/sjar/2013111-3461

来源: DOAJ

【 摘 要 】

Eggplant mottled dwarf virus (EMDV, genus Nucleorhabdovirus, family Rhabdoviridae) is transmitted in nature by leafhoppers and its natural host range includes vegetable crops (eggplant, tomato, potato, pepper), ornamentals (pittosporum, honeysuckle, pelargonium) and wild plants (caper, Solanum nigrum). The prevalence of infections is generally very low. EMDV has been demonstrated to be the causal agent of a vein yellowing disease of China rose (Hibiscus rosa-sinensis) in southern Italy. In this work, four locations from Málaga and Granada provinces (southern Spain) were surveyed in 2011 to study the prevalence of EMDV infections in China rose by serological and molecular methods. Overall, EMDV was detected in 77.3% of the samples (33 out of 45 samples tested). Mechanical transmission tests and immunoelectron microscopy confirmed the presence of EMDV. The possible causes of such a high and unexpected prevalence are discussed. The use of molecular hibridization with an EMDV specific riboprobe is proposed for early screening of vegetative propagated China rose plants to avoid dissemination of infected material.

【 授权许可】

Unknown