Quantitative analysis of how Myc controls T cell proteomes and metabolic pathways during T cell activation

eLife

Julia M Marchingo¹  Doreen A Cantrell¹  Linda V Sinclair¹  Andrew JM Howden¹ 

[1] Cell Signalling and Immunology Division, School of Life Sciences, University of Dundee, Dundee, United Kingdom;

关键词: T lymphocyte;    T cell activation;    proteomics;    Myc;    amino acid transport;   

DOI  :  10.7554/eLife.53725

来源: DOAJ

【 摘 要 】

T cell expansion and differentiation are critically dependent on the transcription factor c-Myc (Myc). Herein we use quantitative mass-spectrometry to reveal how Myc controls antigen receptor driven cell growth and proteome restructuring in murine T cells. Analysis of copy numbers per cell of >7000 proteins provides new understanding of the selective role of Myc in controlling the protein machinery that govern T cell fate. The data identify both Myc dependent and independent metabolic processes in immune activated T cells. We uncover that a primary function of Myc is to control expression of multiple amino acid transporters and that loss of a single Myc-controlled amino acid transporter effectively phenocopies the impact of Myc deletion. This study provides a comprehensive map of how Myc selectively shapes T cell phenotypes, revealing that Myc induction of amino acid transport is pivotal for subsequent bioenergetic and biosynthetic programs and licences T cell receptor driven proteome reprogramming.

【 授权许可】

Unknown