Acute Aerobic Exercise Remodels the Adipose Tissue Progenitor Cell Phenotype in Obese Adults

Frontiers in Physiology

Lindsey A. Muir¹  Carey N. Lumeng²  Kanakadurga Singer²  Cara E. Porsche⁴  Emily M. Krueger⁵  Benjamin J. Ryan⁵  Toree C. Baldwin⁵  Alison C. Ludzki⁵  Michael W. Schleh⁵  Jeffrey F. Horowitz⁵ 

[1] Department of Computational Medicine and Bioinformatics, Medical School, University of Michigan, Ann Arbor, MI, United States;Department of Molecular and Integrative Physiology, Medical School, University of Michigan, Ann Arbor, MI, United States;Department of Pediatrics, Medical School, University of Michigan, Ann Arbor, MI, United States;Graduate Program in Immunology, Medical School, University of Michigan, Ann Arbor, MI, United States;Substrate Metabolism Laboratory, School of Kinesiology, University of Michigan, Ann Arbor, MI, United States;

关键词: preadipocytes;    exercise;    obesity;    macrophages;    endothelial cells;    T cells;   

DOI  :  10.3389/fphys.2020.00903

来源: DOAJ

【 摘 要 】

Adipose tissue pathology in obese patients often features impaired adipogenesis, angiogenesis, and chronic low-grade inflammation, all of which are regulated in large part by adipose tissue stromal vascular cells [SVC; i.e., non-adipocyte cells within adipose tissue including preadipocytes, endothelial cells (ECs), and immune cells]. Exercise is known to increase subcutaneous adipose tissue lipolysis, but the impact of exercise on SVCs in adipose tissue has not been explored. The purpose of this study was to assess the effects of a session of exercise on preadipocyte, EC, macrophage, and T cell content in human subcutaneous adipose tissue. We collected abdominal subcutaneous adipose tissue samples from 10 obese adults (BMI 33 ± 3 kg/m2, body fat 41 ± 7%) 12 h after a 60 min acute session of endurance exercise (80 ± 3%HRpeak) vs. no acute exercise session. SVCs were isolated by collagenase digestion and stained for flow cytometry. We found that acute exercise reduced preadipocyte content (38 ± 7 vs. 30 ± 13%SVC; p = 0.04). The reduction was driven by a decrease in CD34hi preadipocytes (18 ± 5 vs. 13 ± 6%SVC; p = 0.002), a subset of preadipocytes that generates high lipolytic rate adipocytes ex vivo. Acute exercise did not alter EC content. Acute exercise also did not change total immune cell, macrophage, or T cell content, and future work should assess the effects of exercise on subpopulations of these cells. We conclude that exercise may rapidly regulate the subcutaneous adipose tissue preadipocyte pool in ways that may help attenuate the high lipolytic rates that are commonly found in obesity.

【 授权许可】

Unknown