Compensatory Growth Induced in Zebrafish Larvae afterPre-Exposure to a Microcystis aeruginosa Natural Bloom Extract Containing Microcystins

International Journal of Molecular Sciences

关键词: Cyanobacterial bloom extract;    Natural risk;    Microcystins;    Malformations;    Compensatory growth;   

DOI  :  10.3390/ijms10010133

来源: DOAJ

【 摘 要 】

Early life stage tests with zebrafish (Danio rerio) were used to detect toxic effects of compounds from a Microcystis aeruginosa natural bloom extracton their embryolarval development. We carried out the exposure of developing stages of fish to complex cyanobacterial blooms containing hepatotoxic molecules - microcystins. Fish embryo tests performed with the bloom extract containing 3 mg·L-1 Eq microcystin-LR showed that after 24 h of exposure all fish embryos died. The same tests performed with other diluted extracts (containing 0.3, 0.1 and 0.03 mg·L-1 Eq microcystin-LR) were shown to have an influence on zebrafish development and a large number of embryos showed malformation signs (edema, bent and curving tail). After hatching the larvae were transferred to a medium without toxins to follow the larval development under the new conditions. The specific growth of the pre-exposed larvae was significantly more important than that of the control larvae. This may represent a compensatory growth used to reduce the difference in size with the control fish noted after hatching.

【 授权许可】

Unknown