Compensatory Growth Induced in Zebrafish Larvae after Pre-Exposure to a Microcystis aeruginosa Natural Bloom Extract Containing Microcystins

International Journal of Molecular Sciences

Issam El Ghazali³  Sanaa Saqrane³  Antonio Paulo Carvalho²  Youness Ouahid¹  Brahim Oudra³  Francisca Fernandez Del Campo¹ 

[1] Departamento de Biologia, Laboratorio de Fisiologia Vegetal, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain. E-Mails:;Centro Interdisciplinar de Investigação Marinha e Ambiental, CIIMAR/CIMAR-LA, Rua dos Bragas 289, Porto 4050-123, Portugal. E-Mail:;Department of Biology, Laboratory of Biology and Biotechnology of Microorganisms, Microbiology and Environmental Toxicology Unit, Faculty of Sciences Semlalia Marrakech, University Cadi Ayyad, P.O. Box 2390, Marrakech 40000, Morocco. E-Mails:

关键词: Cyanobacterial bloom extract;    Natural risk;    Microcystins;    Malformations;    Compensatory growth;   

DOI  :  10.3390/ijms10010133

来源: mdpi

【 摘 要 】

Early life stage tests with zebrafish (Danio rerio) were used to detect toxic effects of compounds from a Microcystis aeruginosa natural bloom extract on their embryolarval development. We carried out the exposure of developing stages of fish to complex cyanobacterial blooms containing hepatotoxic molecules - microcystins. Fish embryo tests performed with the bloom extract containing 3 mg·L⁻¹ Eq microcystin-LR showed that after 24 h of exposure all fish embryos died. The same tests performed with other diluted extracts (containing 0.3, 0.1 and 0.03 mg·L⁻¹ Eq microcystin-LR) were shown to have an influence on zebrafish development and a large number of embryos showed malformation signs (edema, bent and curving tail). After hatching the larvae were transferred to a medium without toxins to follow the larval development under the new conditions. The specific growth of the pre-exposed larvae was significantly more important than that of the control larvae. This may represent a compensatory growth used to reduce the difference in size with the control fish noted after hatching.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).