Insights into the Antimicrobial, Antioxidant, Anti-SARS-CoV-2 and Cytotoxic Activities of Pistacia lentiscus Bark and Phytochemical Profile; In Silico and In Vitro Study

Antioxidants

Mervat A. R. Ibrahim¹  Mona Warrad²  Samy Selim³  Afaf Alharthi⁴  Soad K. Al Jaouni⁵  Mohammed S. Almuhayawi⁶  Mohanned T. Alharbi⁷  Basel A. Abdel-Wahab⁸  Amnah Mohammed Alsuhaibani⁹  Khaled Rashed^1,10 

[1] Biochemistry Department, Faculty of Agriculture, Ain Shams University, Cairo 11566, Egypt;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences at Al-Quriat, Jouf University, Al Qurayyat 77425, Saudi Arabia;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72388, Saudi Arabia;Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif 21944, Saudi Arabia;Department of Hematology/Oncology, Yousef Abdulatif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia;Department of Medical Microbiology and Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia;Department of Medical Microbiology and Parasitology, Faculty of Medicine, University of Jeddah, Jeddah 23218, Saudi Arabia;Department of Medical Pharmacology, College of Medicine, Assiut University, Assiut 71515, Egypt;Department of Physical Sport Science, College of Education, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia;Pharmacognosy Department, National Research Centre, 33El Bohouth Str., Dokki, Giza 12622, Egypt;

关键词: Pistacia lentiscus;    foodborne control;    antioxidant;    antibacterial activity;    antifungal activity;    antiviral activity;   

DOI  :  10.3390/antiox11050930

来源: DOAJ

【 摘 要 】

Foodborne infections and antibiotic resistance pose a serious threat to public health and must be addressed urgently. Pistacia lentiscus is a wild-growing shrub and has been utilized for medicinal applications as well as for culinary purposes. The antibacterial and antioxidant activities of P. lentiscus bark in vitro, as well as the phytochemical composition, are the focus of this inquiry. The bark extract of P. lentiscus showed significant antimicrobial activity in experiments on bacteria and yeast isolated from human and food sources. The exposure time for the complete inhibition of cell viability of P. aeruginosa in the extracts was found to be 5% at 15 min. Phytochemical inquiry of the methanol extract demonstrates the existence of carbohydrates, flavonoids, tannins, coumarins, triterpenes, and alkaloids. Deep phytochemical exploration led to the identification of methyl gallate, gallic acid, kaempferol, quercetin, kaempferol 3-O-α-rhamnoside, kaempferol 3-O-β-glucoside, and Quercetin-3-O-β-glucoside. When tested using the DPPH assay, the methanol extracts of P. lentiscus bark demonstrated a high free radical scavenging efficiency. Further, we have performed a molecular modelling study which revealed that the extract of P. lentiscus bark could be a beneficial source for novel flavonoid glycosides inhibitors against SARS-CoV-2 infection. Taken together, this study highlights the Pistacia lentiscus bark methanol extract as a promising antimicrobial and antiviral agent.

【 授权许可】

Unknown