The French press: a repeatable and high-throughput approach to exercising zebrafish (Danio rerio)

PeerJ

Daniel Hesselson¹  Rose E. O’Dea²  Takuji Usui²  Melissa L. Fangmeier²  Shinichi Nakagawa²  Daniel W.A. Noble²  Malgorzata Lagisz² 

[1] Diabetes and Metabolism Division, Garvan Institue of Medical Research, Sydney, New South Wales, Australia;Evolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, Australia;

关键词: Burst swimming;    Endurance test;    Sustained swimming;    Technique;    Circular;   

DOI  :  10.7717/peerj.4292

来源: DOAJ

【 摘 要 】

Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34–0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.

【 授权许可】

Unknown