Ginseng-Angelica-Sansheng-Pulvis Boosts Neurogenesis Against Focal Cerebral Ischemia-Induced Neurological Deficiency

Frontiers in Neuroscience

Chienchih Ke¹  Qian Zhang²  Xiaowei Guan³  Xiang Cao⁵  Yun Xu⁵  Bowen Liu⁶  Yang Li⁷  Cheng Luo⁷  Zhenyan Xia⁷  Yonghua Zhao⁸ 

[1] Biomedical Imaging Research Center, National Yang-Ming University, Taipei, Taiwan;Department of Biotherapy, Shenzhen Luohu People’s Hospital, Shenzhen, China;Department of Human Anatomy and Histoembryology, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing, China;Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung City, Taiwan;Department of Neurology, Drum Tower Hospital, Medical School and The State Key Laboratory of Pharmaceutical Biotechnology, Institute of Brain Science, Nanjing University, Nanjing, China;Department of Neurology, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China;State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine, Macau University of Science and Technology, Macau, China;State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China;

关键词: Ginseng-Angelica-Sansheng-Pulvis;    ischemic stroke;    neurogenesis;    neurological deficiency;    neural differentiation;   

DOI  :  10.3389/fnins.2019.00515

来源: DOAJ

【 摘 要 】

BackgroundThe traditional Chinese medicine Ginseng-Angelica-Shanseng-Pulvis (GASP) has been used to treat stroke for 300 years. This present study investigated if it can induce increases in neurogenesis following cerebral ischemic injury.MethodsRats following middle cerebral artery occlusion were orally treated with high, medium, and low doses of a standardized GASP extract.ResultsAfter 14 days, treatment with GASP improved regional blood flow and infarction volume by magnetic resonance imaging scanning, enhanced Ki67+ expression in the subventricular zone, increased brain-derived neurotrophic factor (BDNF) secretion, Nestin, and bone morphogenetic protein (BMP) 2/4 expressions in the hippocampus in a dose-dependent manner. Interestingly, low-dose treatment with GASP downregulated doublecortin and Notch1 expressions in the hippocampus, as well as upregulated glial fibrillary acidic protein expression in the subgranular zone and hairy and enhancer of split (Hes) 5 expression in the hippocampus, while treatment with middle and high doses of GASP reversed these results. Meanwhile, the consumed time was shortened in the basket test and the adhesive removal test and the spending time on exploring novel objects was prolonged by GASP treatment whose effects were more obvious at day 14 post-ischemia.ConclusionOur study demonstrates that treatment with GASP increases neurogenesis and ameliorates sensorimotor functions and recognition memory. We hypothesize that these effects are thought be mediated by an effect on the BMP2/4 pathway and Notch1/Hes5 signal. Due to its beneficial efficacy, GASP can be recognized as an alternative therapeutic agent for ischemic stroke.

【 授权许可】

Unknown