Extracellular vesicles from medicated plasma of Buyang Huanwu decoction-preconditioned neural stem cells accelerate neurological recovery following ischemic stroke

Frontiers in Cell and Developmental Biology

Cell and Developmental Biology

Yajing Li¹  Waqas Ahmed²  Qiankun Zhang³  Jiajun Huang³  Rong Li³  Lukui Chen³  Jiale Liu³  Yifan Zhang³  Jun Long³  Chenyang Gu³  Jianfeng Zhang³  Hengsen Cai⁴  Yong Hu⁵  Ahsan Ali Khan⁶ 

[1] Affiliated Dongguan Hospital, Southern Medical University (Dongguan People’s Hospital), Guangzhou, China;Department of Neurology, Affiliated Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China;Department of Neurosurgery, Neuroscience Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China;Department of Neurosurgery, The Second People’s Hospital of Pingnan, Pingnan, China;Department of Orthopedics and Traumatology, The University of Hong Kong, Hongkong SAR, China;Section of Neurosurgery, The Aga Khan University, Karachi, Pakistan;

关键词: ischemic stroke;    neural stem cells;    extracellular vesicles;    Buyang Huanwu decoction;    medicated plasma;    neurogenesis;    miRNAs profiles;   

DOI  :  10.3389/fcell.2023.1096329

 received in 2022-11-22, accepted in 2023-02-16,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: The neurological impairment of survivors after ischemic stroke poses a serious risk to their quality of life and health. Effective therapeutic options are still lacking. Neural stem cells (NSCs) promote neurogenesis via secreted extracellular vesicles (NSC-EVs), which would be a potential therapeutic option, but the insufficient quantity of NSC-EVs in vivo restrains clinical application. Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) decoction, is promising to alleviate neurological impairment after ischemic stroke. It was speculated that BHD might promote neurological recovery through the NSC-EVs.Methods: The medicated plasma of BHD (MP-BHD) was prepared to precondition NSCs and isolate EVs (BHD-NSC-EVs). Middle cerebral artery occlusion (MCAO) models and primary NSCs were administered to evaluate the therapeutic effect. Next-generation sequencing was performed to explore the mechanism.Results: The BHD-NSC-EVs more significantly accelerated neurological recovery after MCAO and promoted NSCs proliferation and differentiation than BHD and NSC-EVs alone. MP-BHD enhanced the largescale generation of BHD-NSC-EVs, which encapsulated functional miRNA and may play critical roles in neurogenesis.Discussion: In replacing BHD or NSCs, the preconditioned NSC-EVs present a more efficient therapeutic strategy for ischemic stroke. Based on the clinical efficacy of TCM, the preconditioning of NSC-derived EVs via the MP of TCM herbs would presents a newly promising therapeutic strategy for neurological diseases.

【 授权许可】

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Copyright © 2023 Long, Gu, Zhang, Liu, Huang, Li, Zhang, Li, Ahmed, Zhang, Khan, Cai, Hu and Chen.