Application and Perspectives of MALDI–TOF Mass Spectrometry in Clinical Microbiology Laboratories

Microorganisms

Carlos García-Riestra¹  Eva Torres-Sangiao¹  Cristina Leal Rodriguez² 

[1] Escherichia coli Group, Foundation Institute of Health Research—University Hospital Complex of Santiago de Compostela (FIDIS—CHUS), ES-15706 Santiago de Compostela, Spain;Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark;

关键词: MALDI–TOF MS;    microbial identification;    proteomics;    resistome;    disease biomarkers;   

DOI  :  10.3390/microorganisms9071539

来源: DOAJ

【 摘 要 】

Early diagnosis of severe infections requires of a rapid and reliable diagnosis to initiate appropriate treatment, while avoiding unnecessary antimicrobial use and reducing associated morbidities and healthcare costs. It is a fact that conventional methods usually require more than 24–48 h to culture and profile bacterial species. Mass spectrometry (MS) is an analytical technique that has emerged as a powerful tool in clinical microbiology for identifying peptides and proteins, which makes it a promising tool for microbial identification. Matrix assisted laser desorption ionization–time of flight MS (MALDI–TOF MS) offers a cost- and time-effective alternative to conventional methods, such as bacterial culture and even 16S rRNA gene sequencing, for identifying viruses, bacteria and fungi and detecting virulence factors and mechanisms of resistance. This review provides an overview of the potential applications and perspectives of MS in clinical microbiology laboratories and proposes its use as a first-line method for microbial identification and diagnosis.

【 授权许可】

Unknown