Biosensors | |
Microfluidic-Based Amplification-Free Bacterial DNA Detection by Dielectrophoretic Concentration and Fluorescent Resonance Energy Transfer Assisted |
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Michelle M. Packard1  Maxim Shusteff2  | |
[1]Nanobiosensors Laboratory, Michigan State University, East Lansing, MI 48824, USA | |
[2]Lawrence Livermore National Laboratory, 7000 East Ave., Livermore, CA 94550, USA | |
关键词:
fluorescence resonance energy transfer assisted |
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DOI : 10.3390/bios2040405 | |
来源: mdpi | |
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【 摘 要 】
Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times. The assay described in this work incorporates on-chip dielectrophoretic capture and concentration of bacterial cells, thermal lysis, cell permeabilization, and nucleic acid denaturation and fluorescence resonance energy transfer assisted
【 授权许可】
CC BY
© 2012 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190041469ZK.pdf | 517KB | ![]() |