Application of Transcriptomics to Enhance Early Diagnostics of Mycobacterial Infections, with an Emphasis on Mycobacterium avium ssp. paratuberculosis

Veterinary Sciences

AdP. Koets¹  MarielleH. van den Esker¹ 

[1] Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, 8200 AB Lelystad, The Netherlands;

关键词: mycobacterium;    host-pathogen interactions;    paratuberculosis;    Johne’s disease;    diagnostics;    biomarker;    transcriptomics;   

DOI  :  10.3390/vetsci6030059

来源: DOAJ

【 摘 要 】

Mycobacteria cause a wide variety of disease in human and animals. Species that infect ruminants include M. bovis and M. avium ssp. paratuberculosis (MAP). MAP is the causative agent of Johne’s disease in ruminants, which is a chronic granulomatous enteric infection that leads to severe economic losses worldwide. Characteristic of MAP infection is the long, latent phase in which intermittent shedding can take place, while diagnostic tests are unable to reliably detect an infection in this stage. This leads to unnoticed dissemination within herds and the presence of many undetected, silent carriers, which makes the eradication of Johne’s disease difficult. To improve the control of MAP infection, research is aimed at improving early diagnosis. Transcriptomic approaches can be applied to characterize host-pathogen interactions during infection, and to develop novel biomarkers using transcriptional profiles. Studies have focused on the identification of specific RNAs that are expressed in different infection stages, which will assist in the development and clinical implementation of early diagnostic tests.

【 授权许可】

Unknown