AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design

Genome Biology

Bruce R. Conklin¹  Sean Whalen¹  Svetlana Lyalina¹  Michael P. Olvera¹  Katherine S. Pollard¹  Kathleen C. Keough² 

[1] Gladstone Institutes;Pharmaceutical Sciences and Pharmacogenomics Graduate Program, University of California;

关键词: CRISPR;    sgRNA design;    Genomics;    Genome surgery;    Genome editing;    Computational biology;   

DOI  :  10.1186/s13059-019-1783-3

来源: DOAJ

【 摘 要 】

Abstract The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distinguishable by sgRNA designing tools based on one reference genome. AlleleAnalyzer is an open-source software that incorporates single-nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing 1 or multiple haplotypes of a sequenced genome, currently supporting 11 Cas proteins. It also leverages patterns of shared genetic variation to optimize sgRNA design for different human populations. AlleleAnalyzer is available at https://github.com/keoughkath/AlleleAnalyzer.

【 授权许可】

Unknown