Anti-Inflammation Activities of Mycosporine-Like AminoAcids (MAAs) in Response to UV Radiation SuggestPotential Anti-Skin Aging Activity

Marine Drugs

Hyo Hyun Seo¹  Jeong Hun Lee¹  Sang Hyun Moh¹  Hyoung-Shik Kim¹  Mirye Park²  Sung-Suk Suh²  Jinik Hwang²  Taek-Kyun Lee² 

[1] Anti-Aging Research Institute of Bio-FD&C Co. Ltd, Incheon 406-840, Korea;South Sea Environment Research Department, Korea Institute of Ocean Science and Technology, Geoje 656-830, Korea;

关键词: mycosporine-like amino acids (MAAs);    HPLC;    mycosporine-glycine;    shinorine;    porphyra-334;   

DOI  :  10.3390/md12105174

来源: DOAJ

【 摘 要 】

Certain photosynthetic marine organisms have evolved mechanisms to counteract UV-radiation by synthesizing UV-absorbing compounds, such as mycosporine-like amino acids (MAAs). In this study, MAAs were separated from the extracts of marine greenalga Chlamydomonas hedleyi using HPLC and were identified as porphyra-334, shinorine, and mycosporine-glycine (mycosporine-Gly), based on their retention times andmaximum absorption wavelengths. Furthermore, their structures were confirmed by triple quadrupole MS/MS. Their roles as UV-absorbing compounds were investigated in the human fibroblast cell line HaCaT by analyzing the expression levels of genes associatedwith antioxidant activity, inflammation, and skin aging in response to UV irradiation.The mycosporine-Gly extract, but not the other MAAs, had strong antioxidant activity in the 2,2-diphenyl-1-picryhydrazyl (DPPH) assay. Furthermore, treatment with mycosporine-Gly resulted in a significant decrease in COX-2 mRNA levels, which are typically increased in response to inflammation in the skin, in a concentration-dependent manner. Additionally, in the presence of MAAs, the UV-suppressed genes, procollagen C proteinase enhancer (PCOLCE) and elastin, which are related to skin aging, had increased expressionlevels equal to those in UV-mock treated cells. Interestingly, the increased expressionof involucrin after UV exposure was suppressed by treatment with the MAAs mycosporine-Gly and shinorine, but not porphyra-334. This is the first report investigating the biological activities of microalgae-derived MAAs in human cells.

【 授权许可】

Unknown