Journal of Experimental & Clinical Cancer Research | |
Claudin-5 is involved in breast cancer cell motility through the N-WASP and ROCK signalling pathways | |
关键词: Breast cancer; Claudin-5; MDA-MB-231; N-WASP; ROCK; | |
DOI : 10.1186/1756-9966-31-43 | |
来源: DOAJ |
【 摘 要 】
Abstract
Background
Recent studies have shown dysregulation in TJ structure of several cancers including breast. Claudin-5 is a protein member of the TJ structure expressed in both endothelial and epithelial cells. This study examined the level of expression and distribution of Claudin-5 in human breast cancer tissues and the effect of knockdown and forced expression of Claudin-5 in the MDA-MB-231 breast cancer cell line.
Methods
Immunohistochemistry and quantitative-PCR were used to analyse patient tissue samples. The Claudin-5 gene was cloned and overexpressed or knocked down using ribozyme technology in human breast cancer cells. Changes in function were assessed using
Results
Patients whose tumours expressed high levels of Claudin-5 had shorter survival than those with low levels (p = 0.004). Investigating
Conclusions
This study portrays a very new and interesting role for Claudin-5 in cell motility involving the N-WASP signalling cascade indicating a possible role for Claudin-5 in the metastasis of human breast cancer.
【 授权许可】
Unknown