Non-Targeted Dried Blood Spot-Based Metabolomics Analysis Showed Rice Bran Supplementation Effects Multiple Metabolic Pathways during Infant Weaning and Growth in Mali

Nutrients

Hend Ibrahim¹  Annika M. Weber²  Elizabeth P. Ryan³  Hillary V. Smith³  Abdoulaye Bore⁴  Lassina Doumbia⁴  Alima Cissoko⁴  Ousmane A. Koita⁴  Karim Kone⁴  Seydou Douyon⁴  Lansana Sangare⁴  Ababacar Maiga⁴  Anne M. Evans⁵  Kelli Goodman⁵  Brigitte A. Pfluger⁶ 

[1] Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA;Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, CO 80523, USA;Department of Global Health and Health Disparities, Colorado School of Public Health, Colorado State University, Fort Collins, CO 80523, USA;Laboratory of Applied Biology, Science and Technologies Faculty, University of Science, Techniques and Technologies of Bamako, Bamako E 3206, Mali;Metabolon, Inc., Morrisville, NC 27560, USA;Nutrition and Health Sciences, Laney Graduate School, Emory University, Atlanta, GA 30322, USA;

关键词: rice bran;    dried blood spots;    metabolomics;    nutrition;    complementary feeding;    Mali;   

DOI  :  10.3390/nu14030609

来源: DOAJ

【 摘 要 】

Rice bran contains essential nutrients, antioxidants, and bioactives with anti-inflammatory and diarrheal protective properties important for infants. This 6-month randomized controlled trial investigated the effects of heat-stabilized rice bran supplementation during Malian infant weaning. Fifty healthy 6-month-old infants were randomized to a rice bran intervention (N = 25) or non-intervention control group (N = 25). Intervention infants received dose-escalating rice bran supplementation for 6 months (1–5 g/day). Monthly infant dried blood spot and anthropometric measurements were collected. Dried blood spot metabolite abundances were compared monthly according to diet for six months. Supplementation resulted in favorable weight-for-age and weight-for-length z-score changes. Non-targeted dried blood spot-based metabolomics identified 796 metabolites, of which 33% had significant fold differences between groups (7–12 months). Lipids and amino acids represented 70.6% of the metabolites identified. Rice bran supplementation during infant weaning significantly modulated the metabolites involved in antioxidant defenses and with neuroactive properties including reduced glutathione, glycine, glutamate, cysteinylglycine, tryptophan betaine, and choline. These findings support rice bran as a weaning ingredient to meet infant nutritional requirements and with the potential to reduce oxidative stress and improve cognitive outcomes. This study provides evidence for dried blood spots as a cost-effective tool to detect infant biomarkers of nutritional and metabolic status.

【 授权许可】

Unknown