Protocol for generating a mouse model of gastric MALT lymphoma and the identification of MALT lymphoma cell populations by immunostaining

STAR Protocols

Toshiro Sugiyama¹  Yasuyuki Kondo²  Koji Yamamoto³  Naoya Sakamoto⁴ 

[1] Corresponding author;Research Division of Molecular Targeting Therapy and Prevention of GI Cancer, Hokkaido University Hospital, Sapporo, Hokkaido 060-8638, Japan;Department of Gastroenterology and Hepatology, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan;Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Hyogo 650-0017, Japan;

关键词: Cell Biology;    Cancer;    Immunology;    Microbiology;    Microscopy;    Model Organisms;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Helicobacter suis, a zoonotic infection-related bacterium, induces gastric mucosa-associated lymphoid tissue (MALT) lymphoma in humans and animals. However, a lack of suitable animal models complicates the detailed analysis of this disease. Here, we describe the generation of a gastric MALT lymphoma mouse model. We then detail the use of this model combined with an immunostaining protocol to identify the cell populations that constitute gastric MALT lymphoma. This protocol can be used to identify the constituent cells of human MALT lymphoma.For complete details on the use and execution of this profile, please refer to Yamamoto et al. (2021).

【 授权许可】

Unknown