Avermectin induced DNA damage to the apoptosis and autophagy in human lung epithelial A549 cells

Ecotoxicology and Environmental Safety

Cheng Zhang¹  Siyu Wang²  Lin Diao²  Ning Tang²  Lutong Zhang²  Can Wu²  Yang Zhang²  Zhenhai Zhang²  Zhong Li²  Jiagao Cheng²  Liming Tao² 

[1] Department of Pathology, UT southwestern Medical Center, Dallas, TX 75390, United States;Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;

关键词: Avermectin;    Cytotoxicity;    DNA damage;    Programmed cell death;    Human A549 cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Avermectin (AVM), as a biological insecticide, is widely used in agriculture and forestry production globally. However, inhalation of AVM may pose a risk, and the lung is the direct target, but the cytotoxicity of AVM on human lung cells is still unclear. Here, we attempted to elucidate the cytotoxic effect and molecular mechanism of AVM on human lung A549 cells. The results indicated that AVM inhibits cell proliferation, and enhances programmed cell death (apoptosis and autophagy). In addition, we found the AVM-treated cells showed an obvious drop in mitochondrial membrane potential and LC3-I/II, increased ROS production, DNA double-strand breaks, caspase-3/9 activated, PARP cleaved, cytochrome c and Bax/Bcl-2 content rise. The results showed that AVM induced mitochondria-related apoptosis and autophagy in lung A549 cells. These results indicate that AVM can pose a potential threat to human health by inducing DNA damage and programmed cell death.

【 授权许可】

Unknown