Rapid Detection and Quantification of Patulin and Citrinin Contamination in Fruits

Molecules

Sudharsan Sadhasivam¹  Varda Zakin¹  Edward Sionov¹  Omer Barda¹  Ram Reifen² 

[1] Department of Food Sciences, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion 7528809, Israel;The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science and Nutrition, The Hebrew University of Jerusalem, Rehovot 7610001, Israel;

关键词: mycotoxin detection;    pome fruits;    patulin;    citrinin;    HPLC;   

DOI  :  10.3390/molecules26154545

来源: DOAJ

【 摘 要 】

Patulin (PAT) and citrinin (CTN) are the most common mycotoxins produced by Penicillium and Aspergillus species and are often associated with fruits and fruit by-products. Hence, simple and reliable methods for monitoring these toxins in foodstuffs are required for regular quality assessment. In this study, we aimed to establish a cost-effective method for detection and quantification of PAT and CTN in pome fruits, such as apples and pears, using high-performance liquid chromatography (HPLC) coupled with spectroscopic detectors without the need for any clean-up steps. The method showed good performance in the analysis of these mycotoxins in apple and pear fruit samples with recovery ranges of 55–97% for PAT and 84–101% for CTN, respectively. The limits of detection (LOD) of PAT and CTN in fruits were 0.006 µg/g and 0.001 µg/g, while their limits of quantification (LOQ) were 0.018 µg/g and 0.003 µg/g, respectively. The present findings indicate that the newly developed HPLC method provides rapid and accurate detection of PAT and CTN in fruits.

【 授权许可】

Unknown