A Gold Nanoparticle and Aflatoxin B1-BSA Conjugates Based Lateral Flow Assay Method for the Analysis of Aflatoxin B1

Materials

Jihea Moon²  Giyoung Kim¹ 

[1] Post-Harvest & Food Engineering Division, Department of Agricultural Engineering, National Academy of Agricultural Sciences, Suwon 441-707, Korea;

关键词: aflatoxin B1;    gold nanoparticle;    immune-chromatographic assay;    dot strip assay;    mycotoxin detection;   

DOI  :  10.3390/ma5040634

来源: mdpi

【 摘 要 】

A rapid and simple immuno-chromatographic assay was developed to detect aflatoxin B1 (AFB1). The assay was based on a modified competitive binding format using colloidal gold and polyclonal antibody (Pab) conjugates. The anti-AFB1 Pab was immobilized to a defined detection zone on a porous nitrocellulose membrane and colloidal gold particles were conjugated to AFB1-BSA which served as a detection reagent. The AFB1-containing sample was added to the membrane and allowed to move with AFB1-BSA-coated particles dried on the conjugation pad. The mixture was then passed along the porous membrane by capillary action past the Pab in the detection zone, which captured AFB1 or AFB1-BSA. AFB1 in the sample inhibits binding of AFB1-BSA conjugated gold particles to the Pab and prevents formation of a red color dot. In the absence of AFB1, AFB1-BSA conjugated gold particles bound to the Pab, give a red color within this detection zone. With this method, 10 μg/mL of AFB1 was detected in less than 10 min. The developed AFB1 assay also showed no cross reaction to Ochratoxin A (OTA).

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.