Ratiometric Polymer Probe for Detection of Peroxynitrite and the Application for Live-Cell Imaging

Molecules

Chunfei Wang¹  Jingyun Tan¹  Xuanjun Zhang¹  HioKuan Lao¹ 

[1] Cancer Centre and Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau SAR 999078, China;

关键词: ratiometric;    fluorescent probe;    polymer probe;    peroxynitrite;    cell imaging;   

DOI  :  10.3390/molecules24193465

来源: DOAJ

【 摘 要 】

Peroxynitrite (ONOO⁻) is one of the sources of oxidation stress involved in many biological signaling pathways. The role of ONOO⁻ being a double-edged sword in biological systems drives the development of effective detection tools. In this work, a boronate-based polymeric fluorescent probe PB-PVA was synthesized and the probe performance was evaluated. The probe exhibits ratiometric sensing of ONOO⁻ in a range of 0−6 µM. There is good linear relationship between the probe fluorescence intensity ratio and ONOO⁻ concentration. The probe also displays moderate selectivity towards ONOO⁻ over other ROS. Moreover, it is water-soluble and possesses good biocompatibility which aids the imaging of ONOO⁻ in living cells. These properties could make the probe a promising tool in in vitro study related to ONOO⁻.

【 授权许可】

Unknown